This kit uses a cell-permeable green fluorescent Cyto-dye (Ex. max.: 488 nm; Em. max.: 518 nm) to stain live cells, and propidium iodide (Ex. max.: 488 nm; Em. max.: 615 nm) to stain dead cells. Stained live and dead cells can be visualized by fluorescence microscopy using a band-pass filter which detects FITC and rhodamine. Viable cells will stain only with the Cyto-dye, fluorescing green, whereas the dead cells will stain with both Cyto-dye (green) and propidium iodide (red), resulting in a yellow fluorescence. Note: 1 T = 1 test.
Optimal staining conditions may vary among different cell types and should be determined empirically by the investigator.
Catalogue Number
QIA76
Brand Family
Calbiochem®
References
References
Luther, E., and Kamenstsky, L.A. 1996.Cytometry23,272. Frey. T., et al. 1995.Cytometry21,265.
Product Information
Detection method
Fluorescence
Form
100 Tests
Format
Fluorescence microscopy
Kit contains
Staining Buffer, Cyto-Dye, Propidium Iodide, and a user protocol.
Biological Information
Assay time
0.5 h
Sample Type
Cell suspensions or adherent cells
Species
a broad range of species
Safety Information
R Phrase
R: 36/37/38-46
Irritating to eyes, respiratory system and skin. May cause heritable genetic damage.
S Phrase
S: 26-36/37/39-45
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing, gloves and eye/face protection. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Intended use
The Calbiochem®Live/Dead Double Staining Kit can be used to distinguish between live and dead cells, which is essential for the study of growth control and cell death.
Storage and Shipping Information
Ship Code
Dry Ice Only
Toxicity
Multiple Toxicity Values, refer to MSDS
Storage
-20°C
Storage Conditions
Upon arrival, store the entire contents of the kit at -20°C. Following initial use of the Staining Buffer, refrigerate (4°C) the remaining buffer.
Protect from Moisture
Protect from moisture
Do not freeze
No
Supplemental Information
Kit contains
Staining Buffer, Cyto-Dye, Propidium Iodide, and a user protocol.
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